Process of preparing and antiberiberic.



UN ED" STATES PATENT oFFioE.

JINNOSUKE TSUZUKI, or oorxuno, NEAn'roxYo, JAPAN.

PROCESS OF PREPARING .8-1 AN-TIBERIBERIC.

No Drawing.

,specifleatton'of Letters IEatent. Patented Apr. 15,1913. Applicationfiled January 13, 1912, Serial No. 671,1i9.

To all whom it may concern Be it known that I, JmNosUxn Tsozmn, asubject of the Emperor of Japan, residing at Og'ikubo, near Tokyo,Japan, have 1nvented'certain new and useful Improvements in Processes ofPreparing an Antiberiberlc, of which the following is a. specification.

This invention consists in the production of an antiberibericpreparation eflicaciousfor the treatment of beriberi from rice-bran byextracting the rice-bran with alcohol to form an alcohol extract,evaporating or concentrat-ing this extract at a. low temperature,

dissolving the concentrated extract in water, separating impurities bytreatment with am- 'monium sulfate, and separating the desired extractedmaterial from .the purified aqueous solution by alcohol to forman'alcohol solution of the purified product, from which a concentratedproduct can be obtained by evaporation at a low temperature of thealcohol extract, and by separation of. the ammonium sulfate contained inthe solution.

.The process of producing this product is more fully described by thefollowing description, which is to be considered as illus-- tratingratherthan as limiting it. To 1000 g. of rice-bran 2000 g. ofalcohol ofabout 60% "strength are added and the mixture is allowed to stand forabout twelve hours with occasional stirring ata temperature of 10 to 20C. It is then filtered and pressed, a cloth filter being suitable forthis, purpose. The filtrate and liquor resulting from pressing may befiltered again, if necessary, and

amount to about 1500 g. of a transparent yellow liquid. This liquid. isthe alcohol extract of the rice-bran and forms the raw material of thepresent process.

The extract is evaporated at the'temperature of 60 to 80 0, and abrownish thick syrupy liquid (about 200 grams) is obtained. This isdissolved in 2000 grams of ordinary water, then ammonium sulfate isslowly put in while stirring. The sulfate dissolve-s, and when theliquidis saturated with the sulfate it will be precipitated at the bottom ofthe vessel.- As soon as this is noticed the adding of the sulfate isstopped. Though it is not easy to accurately determine the quantity ofthe sulfate required, it maybe stated that more than rams are usuallyadded to 2000- of the liquid.

When the liquid is saturated withthe sulfate the albumin contained inthe liquid is separate and comes to thesurface. he

on the surface and does not IIllX with the liquid, this phenomenon beingdue to the fact thatthe absorbing power of the sulfate and water isgreater than that of alcohol and water. The alcohol forming the upperlayer always absorbs'a certain definite quantity of water and theammonium sulfate contained in it, and this whether the alcohol used isof 60 or 90% purity. While the watery and alcohol layers are in contactwith each other the soluble matters are transmitted from the waterylayers to the alcohol layer, with the result that the latter graduallybecomes tinged in color. The two'layers are left in'contaot for about anhour and then with a funnel the alcohol layer is separated, The beriberispecific being soluble in alcohol is held in the separated layer.

The alcoholisfreed by filtering from insoluble albuminous matters andother foreign ingredients which are found more or less in it,'and atransparent reddish brown liquid is obtained. When subjected toevaphydrated alcohol remains in it. The beriberi specific being solubleis held in this dehydrated alcohol; When filtered, a transparent reddishbrown liquid is obtained, and this is subjected to evaporation at thetemperature of 60 to 80 C., resulting in brownish 'thick juice weighingabout 50 grams. This is called the antiberiberic extract and contains inconstant state a large proportion of pure antiberiberic which is highlyeflicacious in the treatment of beriberi.

The specific ingredient of rice-bran which is thus obtained in the formof an extracthas been :found efficacious for treating beriberi, its useand action being in many respects similar to those of diphtheriaantitoxin in the treatment of diphtheria.

,, The extract above referred to hasan acid reaction and is hygroscopicso that it does not readily harden -unl left in a thin layer ina-desiccator for a considerable period of time, It can be administeredhypodermically in dilute solution. a

in the production of this product it'has been found that alkalis andacids should be avoided, as well as hi h temperatures. Usually atemperature be ow 80 C. is desirable.

I clalim:

1. The process of y from rice-bran suitable for the treatment ofberiberi which comprises extracting ricebran withalcohol-,concentrat-ing the alcoholextract, purifying the concentratedextract by treatment with ammonium sulfate in aqueous solution,extracting the purified aqueous solution with alcohol, concentrating,extracting the concentrated material with absolute alcohol and thencondensing the ex tract at a low temperature.

' 2. In the production of preparing a product a product from rice-bransuitable for the treatment of beriberi the step which comprisespurifying-an aqueous solution of alcohol-soluble extractive matter bytreatment with ammonium sulfate.

'3. In the production of a product from rice-bran suitable for thetreatment of heriberi the step which comprises separating the purifiedproduct from admixed ammonium sulfate by extraction with absolutealcohol.

a, The process of preparing a product from rice-bran suitable for thetreatment of beriberi which comprises extracting ricebraln with analcoholic organic solvent, and purifying the extracted material fromaccompanying albuminous impurities.

In testimony whereof I afi'ix my signature 40 in presence of twowitnesses.

JINNOSUKE TSUZUKI.

Witnesses: Y J. K. CALDWELL,

M. NAMBU.

